Hif 1a antibody
WebHIF-1 alpha Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocyte organoids (Cat no:20960-1-AP) FH. Christian (Verified Customer) (01-07-2024) Unfortunately much background an many unspecific bands in lysates of human chondrocytes and BV2 microclia cells (less unspecific bands in rat chondrocytes). WebThe primary antibodies anti-HIF1α ( 20960-1-AP) and anti-TRIM14 ( 15742-1-AP) were purchased from ProteinTech Group (Chicago, IL, United States). Protein May 16, 2024 Xu Jing, Lutao Du, Shuang Shi, Aijun Niu, Jing Wu, Yunshan Wang, Chuanxin Wang. (19) A novel hypoxia-driven gene signature that can predict the prognosis of hepatocellular ...
Hif 1a antibody
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WebView our 36 HIF-1 alpha/HIF1A products for your research including HIF-1 alpha/HIF1A Primary Antibodies, Small Molecules, Proteome Profiler Antibody Arrays, Peptides, ELISAs, and cDNA Clones. Web11 de abr. de 2024 · HIF-1α activates TMEM237 transcription in hypoxic HCC cells. We previously investigated hypoxia-induced transcripts in Hep3B cells using a microarray assay [].The microarray data (GSE155505) showed that TMEM237 was significantly upregulated in Hep3B cells under hypoxic conditions (Fig. 1a). Then, RT‒qPCR and Western blotting …
WebDescription: This ESEE122 monoclonal antibody reacts with hypoxia inducible factor-1 alpha. The hypoxia inducible factor (HIF-1) is a transcription factor composed of an inducible alpha form and the … Web保存条件: HIF-1alpha/HIF1A Mouse Monoclonal Antibody -20ºC保存,需避免反复冻融。Western一抗稀释液-20℃或4℃保存,一年有效,Western一抗稀释液优先推荐4℃保 …
Web保存条件: HIF-1alpha/HIF1A Mouse Monoclonal Antibody -20ºC保存,需避免反复冻融。Western一抗稀释液-20℃或4℃保存,一年有效,Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
WebProduct Description. Hypoxia-inducible factor- 1α (HIF- 1α) is a transcription factor that accumulates under low- oxygen conditions. 1, 2 Following hypoxic stimulus and cytoplasmic accumulation, HIF- 1α migrates to the nucleus where with other transcription factors, it drives the production of stress- adaptive proteins. dairy cip temperatureWebI've had good luck with HIF-1a antibody Cat#3716 from Cell Signaling Tech. As previously suggested hypoxic chamber helps, however, if you do it quickly you don't need the chamber. bioprofarma bago s.aWebDetection of Human HIF‑1 alpha /HIF1A by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 150 µM CoCl 2 for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat HIF-1a/HIF1A Antigen-affinity Purified Polyclonal Antibody, followed by HRP-conjugated Anti … dairy chinaWebHIF-1 alpha Antibody detects endogenous levels of total HIF-1 alpha. Immunogen: A synthesized peptide derived from human HIF-1 alpha. Description: Hypoxia-inducible … bioproffaWebWB recognizes a band in hypoxic samples at ~115 kDa. HIF-1 alpha protein (NBC1-18422) has been successfully used for antibody preadsorption/blocking assay in IHC-P with HIF-1 alpha antibody NB100-479. Flow Cytometry usage reported in scientific literature (PMID 21917971). Use in Immunoblotting reported in scientific literature (PMID 28506759). bioprofile assay data sheetWebTo determine whether HIF-1α binds these HREs, we performed a CHIP assay using anti-HIF-1α antibodies in A549 cells cultured under hypoxic (1.5% O 2) or normoxic (21% O 2) conditions. DNA fragments containing the HRE1 and/or HRE2 binding sites were significantly more abundant under hypoxic conditions ( Figure 4G ), suggesting that HIF … bioprofile 400 reagent pack sdsWebHIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of … bio products ltd