Hif 1a antibody

WebDetection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line treated with CoCl 2 was stained with Mouse Anti-Human/Mouse HIF-1a PE-conjugated Monoclonal Antibody (Catalog # IC1935P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram).To facilitate intracellular staining, … WebLane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti- Human HIF-1α antibody. Show More Show Less ... (HIF-1). HIF-1 is a heterodimer whose α and β subunits are members of the PAS family of basic helix-loop-helix (bHLH) transcription factors.

HIF-1 alpha antibody from Proteintech Group Inc - Biocompare

WebCytoplasmic in normoxia, nuclear translocation in response to hypoxia. Specificity. This antibody is directed against human HIF-1a hydroxyP564 and is specific for the … WebHIF-1α Antibody 可检测 HIF-1α 总蛋白的内源水平。 该抗体在生理条件不与其他家族成员发生交叉反应,并且不会检测显著水平的羟化 HIF-1α。 物种反应性: 人, 猴 来源/纯化 使 … bio products laboratory wd6 3bx https://mariamacedonagel.com

HIF-1alpha/HIF1A Mouse Monoclonal Antibody (HIF …

WebHIF1A Polyclonal Antibody, Invitrogen™-Rabbit Polyclonal Antibody Achetez HIF1A Polyclonal Antibody, Invitrogen™ sur Fishersci.fr Français ... HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) ... WebHIF-1 alpha Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocyte organoids (Cat no:20960-1-AP) FH. Christian (Verified Customer) (01-07-2024) … WebKnockout Validated Rabbit Polyclonal anti-HIF-1 alpha Antibody - BSA Free. Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, Dual ISH-IHC + more. Reactivity: … bioprofe

HIF-1α Antibody Cell Signaling Technology

Category:Hypoxia markers guide Abcam

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Hif 1a antibody

Anti-HIF-1 alpha antibody [ESEE122] (ab8366) Abcam

WebHIF-1 alpha Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocyte organoids (Cat no:20960-1-AP) FH. Christian (Verified Customer) (01-07-2024) Unfortunately much background an many unspecific bands in lysates of human chondrocytes and BV2 microclia cells (less unspecific bands in rat chondrocytes). WebThe primary antibodies anti-HIF1α ( 20960-1-AP) and anti-TRIM14 ( 15742-1-AP) were purchased from ProteinTech Group (Chicago, IL, United States). Protein May 16, 2024 Xu Jing, Lutao Du, Shuang Shi, Aijun Niu, Jing Wu, Yunshan Wang, Chuanxin Wang. (19) A novel hypoxia-driven gene signature that can predict the prognosis of hepatocellular ...

Hif 1a antibody

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WebView our 36 HIF-1 alpha/HIF1A products for your research including HIF-1 alpha/HIF1A Primary Antibodies, Small Molecules, Proteome Profiler Antibody Arrays, Peptides, ELISAs, and cDNA Clones. Web11 de abr. de 2024 · HIF-1α activates TMEM237 transcription in hypoxic HCC cells. We previously investigated hypoxia-induced transcripts in Hep3B cells using a microarray assay [].The microarray data (GSE155505) showed that TMEM237 was significantly upregulated in Hep3B cells under hypoxic conditions (Fig. 1a). Then, RT‒qPCR and Western blotting …

WebDescription: This ESEE122 monoclonal antibody reacts with hypoxia inducible factor-1 alpha. The hypoxia inducible factor (HIF-1) is a transcription factor composed of an inducible alpha form and the … Web保存条件: HIF-1alpha/HIF1A Mouse Monoclonal Antibody -20ºC保存,需避免反复冻融。Western一抗稀释液-20℃或4℃保存,一年有效,Western一抗稀释液优先推荐4℃保 …

Web保存条件: HIF-1alpha/HIF1A Mouse Monoclonal Antibody -20ºC保存,需避免反复冻融。Western一抗稀释液-20℃或4℃保存,一年有效,Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。

WebProduct Description. Hypoxia-inducible factor- 1α (HIF- 1α) is a transcription factor that accumulates under low- oxygen conditions. 1, 2 Following hypoxic stimulus and cytoplasmic accumulation, HIF- 1α migrates to the nucleus where with other transcription factors, it drives the production of stress- adaptive proteins. dairy cip temperatureWebI've had good luck with HIF-1a antibody Cat#3716 from Cell Signaling Tech. As previously suggested hypoxic chamber helps, however, if you do it quickly you don't need the chamber. bioprofarma bago s.aWebDetection of Human HIF‑1 alpha /HIF1A by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 150 µM CoCl 2 for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat HIF-1a/HIF1A Antigen-affinity Purified Polyclonal Antibody, followed by HRP-conjugated Anti … dairy chinaWebHIF-1 alpha Antibody detects endogenous levels of total HIF-1 alpha. Immunogen: A synthesized peptide derived from human HIF-1 alpha. Description: Hypoxia-inducible … bioproffaWebWB recognizes a band in hypoxic samples at ~115 kDa. HIF-1 alpha protein (NBC1-18422) has been successfully used for antibody preadsorption/blocking assay in IHC-P with HIF-1 alpha antibody NB100-479. Flow Cytometry usage reported in scientific literature (PMID 21917971). Use in Immunoblotting reported in scientific literature (PMID 28506759). bioprofile assay data sheetWebTo determine whether HIF-1α binds these HREs, we performed a CHIP assay using anti-HIF-1α antibodies in A549 cells cultured under hypoxic (1.5% O 2) or normoxic (21% O 2) conditions. DNA fragments containing the HRE1 and/or HRE2 binding sites were significantly more abundant under hypoxic conditions ( Figure 4G ), suggesting that HIF … bioprofile 400 reagent pack sdsWebHIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of … bio products ltd