Phosphinothricin n-acetyltransferase
WebApr 3, 2024 · In this study, we used GC/MS to analyze polar and nonpolar secondary metabolites for the evaluation of unintended changes to chemical compositions in RR, compared with DJ. For example, RR is herbicide-tolerant because one of its selection markers is a bar gene encoding a phosphinothricin- N -acetyltransferase enzyme. WebL-Phosphinothricin (L-Pt)-resistant plants were constructed by introducing a modified phosphinothricin-N-acetyl-transferase gene (pat) via Agrobacterium-mediated gene …
Phosphinothricin n-acetyltransferase
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WebDroege, W.; Broer, I.; Puehler, A.: Transgenic plants containing the phosphinothricin-N-acetyltransferase gene metabolize the herbicide l-phosphinothricin (glufosinate) … WebNov 27, 2024 · Abstract Bialaphos resistance (BAR) and phosphinothricin acetyltransferase (PAT) genes, which convey resistance to the broad-spectrum herbicide phosphinothricin (also known as glufosinate) via N-acetylation, have been globally used in basic plant research and genetically engineered crops.
WebJun 1, 2014 · In this work, a novel phosphinothricin N-acetyltransferase (RePAT) from the marine bacterium Rhodococcus sp. was obtained. The RePAT can utilize both PPT and l … WebJun 1, 2014 · Phosphinothricin Phosphinothricin-N-acetyltransferase Cold-adapted enzyme 1. Introduction Recently, much attention has been focused on the enzymatic modification of nonselective herbicides, owing to the potential application of the genes encoding such enzymes in agricultural biotechnology.
WebGlufosinate (also known as phosphinothricin and often sold as an ammonium salt) is a naturally occurring broad-spectrum herbicide produced by several species of Streptomyces soil bacteria. Glufosinate is a non-selective, contact herbicide, with some systemic action. Plants may also metabolize bialaphos and phosalacine, other naturally occurring …
WebThe phosphinothricin acetyltransferase (PAT) proteins, which are encoded by the bar coding sequence from Streptomyces hygroscopicus or the pat coding sequence from Streptomyces viridochromogenes, are present in glufosinate-ammonium-tolerant plant varieties of various crops such as corn, cotton, rice, oilseed rape, and soybean.
WebMay 26, 2024 · PAT protein overexpression and purification. PAT (phosphinothricin acetyltransferase) expression condition were varied to obtain high yield, and transformed E. coli was examined in various condition such as temperature, IPTG concentration and culturing time. IPTG concentration of 0, 0.5 and 1 mM concentration were used at 18 °C, … highlights magazine pay onlineWebResistance to bialaphos, a non-selective herbicide, was intro duced into cotton through genetic engineering. A gene encoding phosphinothric in acetyltransferase (bar) from Streptomyces hygroscopicus was inserted into elite varieties of cotton through particle bombardment. Based on the marker gene, β-glucuronidase (gus) expression, a total of 18 … small portable buildings garageWebMar 20, 2006 · Similarly, bar and pat genes isolated from Streptomyces hygroscopicus and S. viridochromogenes, respectively, have been inserted into plants to encode phosphinothricin N -acetyltransferase to detoxify glufosinate. Glufosinate-tolerant crops have been commercialized using one of these two transgenes. Download to read the full … small portable buildingsWebGmGSTU23 was then linearly cloned into the pTF101.1 binary vector with a modified CaMV 35S promoter, which contained a phosphinothricin acetyltransferase (bar) resistance … highlights magazine picture findWebNational Center for Biotechnology Information small portable business projectorWebBiologically, phosphinothricin is a structural mimic of γ-glutamyl phosphate and an inhibitor of glutamine synthetase. 24 Phosphinothricin is first phosphorylated by Gln synthetase, and then the phosphorylated intermediate inactivates the enzyme. 24,25 Gln synthetase inhibition results in accumulation of glutamate and ammonia, with the latter … small portable buildings with bathroomsPhosphinothricin acetyltransferase (EC 2.3.1.183, PAT, PPT acetyltransferase, Pt-N-acetyltransferase, ac-Pt) is an enzyme with systematic name acetyl-CoA:phosphinothricin N-acetyltransferase. This enzyme catalyses the following chemical reaction acetyl-CoA + phosphinothricin CoA + N-acetylphosphinothricin highlights magazine promo code